The Rabbit Polyclonal anti-MPI antibody has been validated for WB, IF, IP, ID, EIA, RIA and DB. It is suitable to detect MPI in samples from Saccharomyces cerevisiae.
IgG fraction of polyclonal rabbit antiserum to phosphomannose isomerase from baker's yeast. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
Purification
Ammonium Sulphate Precipitation and Ion Exchange Chromatog
Immunogen
Phosphomannose isomerase isolated and purified from baker’s yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure