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PHKB antibody
The Sheep Polyclonal anti-PHKB antibody has been validated for WB, IP, IF, DB, EIA, ID and RIA. It is suitable to detect PHKB in samples from Rabbit.
Quick Overview for PHKB antibody (ABIN568400)
Target
See all PHKB Antibodies
PHKB
(Phosphorylase Kinase, beta (PHKB))
Reactivity
All reactivities for PHKB antibodies
Rabbit
Host
All hosts for PHKB antibodies
Sheep
Clonality
All clonalities for PHKB antibodies
Polyclonal
Conjugate
All conjugates for PHKB antibodies
This PHKB antibody is un-conjugated
Application
All applications for PHKB antibodies
Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Dot Blot (DB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Radioimmunoassay (RIA)
Product Details anti-PHKB Antibody
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Specificity
IgG fraction of polyclonal sheep antiserum to phosphorylase b from rabbit muscle. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
Purification
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Immunogen
Phosphorylase b isolated and purified from rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Isotype
IgG
Alternatives
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Application Details
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Application Notes
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Handling
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Reconstitution
Restore by adding 1.0 mL of sterile distilled water
Concentration
10,0 mg/mL
Buffer
PBS, pH 7.2 without preservatives and foreign proteins
Preservative
Without preservative
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Prior to and following reconstitution store the antibody at 2-8 °C for one month or at -20 °C for longer.
Target Details for PHKB
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Target
PHKB
(Phosphorylase Kinase, beta (PHKB))
Alternative Name
PHKB
Background
Synonyms: Phosphorylase b kinase regulatory subunit beta, Phosphorylase kinase subunit beta
Gene ID
100009137
NCBI Accession
NP_001075770
UniProt
P12798
Pathways
Cellular Glucan Metabolic Process
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