Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to ribonuclease A from bovine pancreas. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
Characteristics
Molar Ratio: Biotin/IgG ~4.7
Purification
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Immunogen
Ribonuclease A isolated and purified from bovine pancreas. Freund’s complete adjuvant is used in the first step of the immunization procedure.