CCL13 antibody

Catalog No. ABIN5687594

This anti-CCL13 antibody is a Goat Polyclonal antibody detecting CCL13 in WB, ELISA and Neut. Suitable for Human.

Quick Overview for CCL13 antibody (ABIN5687594)

Target: CCL13 (Chemokine (C-C Motif) Ligand 13 (CCL13))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CCL13 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CCL13 Antibody

Purification: Anti-hMCP-4 specific antibody was purified by affinity chromatography employing immobilized hMCP-4 matrix.

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hMCP-4 (human Macrophage/Monocyte chemotactic protein-4).

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hMCP-4 (30.00 ng/mL), a concentration of 3.0 - 4.0 μ,g/mL of this antibody is required.

ELISA:

To detect hMCP-4 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMCP-4.

Sandwich:

To detect hMCP-4 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human MCP-4 (XP-5225Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMCP-4.

Western Blot:
To detect hMCP-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: MCP-4 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for CCL13

Target: CCL13 (Chemokine (C-C Motif) Ligand 13 (CCL13))

Alternative Name: MCP-4

Background: MCP4 is a member of the b-chemokine family (C-C) of Cytokines. Like Eotaxin, MCP4 is a powerful eosinophil chemo-attractant. Additionally, like MCP-3, MCP-4 elicits chemo-attraction of monocytes and T lymphocytes

Gene ID: 6357

UniProt: Q99616

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, The Global Phosphorylation Landscape of SARS-CoV-2 Infection