CCL24 antibody

Catalog No. ABIN5687642

This anti-CCL24 antibody is a Goat Polyclonal antibody detecting CCL24 in WB, ELISA and Neut. Suitable for Mouse.

Quick Overview for CCL24 antibody (ABIN5687642)

Target: CCL24 (Chemokine (C-C Motif) Ligand 24 (CCL24))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This CCL24 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CCL24 Antibody

Purification: Anti-murine Eotaxin-2 specific antibody was purified by affinity chromatography employing immobilized murine Eotaxin-2 matrix.

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant murine Eotaxin-2.

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mEotaxin-2 (100 ng/mL), a concentration of 7.0-8.0 μ,g/mL of this antibody is required.

ELISA:

To detect murine Eotaxin-2 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant murine Eotaxin-2.

Sandwich: To detect mEotaxin-2 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine Eotaxin-2 (XP-5125Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mEotaxin-2.

Western Blot:
To detect murine Eotaxin-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant murine Eotaxin-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: Eotaxin-2 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for CCL24

Target: CCL24 (Chemokine (C-C Motif) Ligand 24 (CCL24))

Alternative Name: Eotaxin-2

Background: Eotaxin is a 74 amino acid, eosinophil chemotactic CC chemokine originally found in bronchoalveolar lavage fluid from allergic inflammatory subjects. It is involved in regulating the recruitment and activation of inflammatory leukocytes, particularly eosinophils. Eotaxin binds and activates the CCR3 chemokine receptor, and may play a fundamental role in the development of allergic responses.

Gene ID: 56221

UniProt: Q9JKC0

Pathways: Regulation of Actin Filament Polymerization