IL-20 antibody (Biotin)

Catalog No. ABIN5688339

This Goat Polyclonal antibody specifically detects IL-20 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for IL-20 antibody (Biotin) (ABIN5688339)

Target: IL-20 (IL20) (Interleukin 20 (IL20))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This IL-20 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-IL-20 Antibody (Biotin)

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hIL-20. Human IL-20 specific antibody was purified by affinity chromatography and then biotinylated.

Application Details

Application Notes: ELISA:
Direct:
To detect hIL-20 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-20.

Sandwich
To detect hIL-20 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human IL-20 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-20.

Western Blot:
To detect hIL-20 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-20 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: IL-20 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for IL-20

Target: IL-20 (IL20) (Interleukin 20 (IL20))

Alternative Name: IL-20

Background: IL20 is a cytokine structurally related to interleukin 10 (IL10). This cytokine has been shown to transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. A specific receptor for this cytokine is expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis.

Gene ID: 50604

UniProt: Q9NYY1

Pathways: Protein targeting to Nucleus