CCL8 antibody

Catalog No. ABIN5688696

This Mouse Monoclonal antibody specifically detects CCL8 in WB, ELISA and Neut. It exhibits reactivity toward Human.

Quick Overview for CCL8 antibody (ABIN5688696)

Target: CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCL8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Clone: 1-1_2D4-1A3

Product Details anti-CCL8 Antibody

Purification: The monoclonal antibody against human MCP-2 designated as VL-1 was purified from ascites fluid on a Protein A affinity column.

Immunogen: The monoclonal antibody was derived form BALB/c X ICR F1 mice and belongs to the IgG1K isotype.

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hMCP-2 (100 ng/mL), a concentration of 0.8-1.0 μ,g/mL of this antibody is required.

ELISA:

In a sandwich ELISA (assuming 100 μ,L/well), a concentration of 1.0-2.0 μ,g/mL of this antibody will detect at least 100 pg/mL of recombinant human MCP-2 when used with our biotinylated antigen affinity purified anti-human MCP-2 (XP-5224Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μ,g/mL.

Western Blot:

To detect hMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-2 is 0.25-0.50 ng/lane, under non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: 4 °C,-20 °C

Storage Comment: MCP-2 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.

Target Details for CCL8

Target: CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

Alternative Name: MCP-2

Background: MCP 2 is a chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. MCP 2 is important to inflammatory host responses, and is found in the highest concentration in the small intestine and peripheral blood cells.

Gene ID: 6355

UniProt: P80075