BMP4 antibody

Catalog No. ABIN5688733

The Mouse Monoclonal anti-BMP4 antibody has been validated for WB, ELISA and Neut. It is suitable to detect BMP4 in samples from Human.

Quick Overview for BMP4 antibody (ABIN5688733)

Target: BMP4 (Bone Morphogenetic Protein 4 (BMP4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BMP4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Clone: 3H2

Product Details anti-BMP4 Antibody

Purification: This IgG2bK antibody was purified from cell culture by Protein G affinity chromatography.

Immunogen: BMP-4 antibody is produced in mice using highly pure (>98%) recombinant human BMP-4 as the immunizing antigen.

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hBMP-4 (5.0 ng/mL), a concentration of 0.4-0.6 μ,g/mL of this antibody is required.

ELISA:

In a sandwich ELISA (assuming 100 μ,L/well), a concentration of 4.0-8.0 μ,g/mL of this antibody will detect at least 2000 pg/mL of recombinant human BMP-4 when used in conjunction with compatible secondary reagents.

Western Blot:
To detect hBMP-4 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-4 is 2.0-4.0 ng/lane, under non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: 4 °C,-20 °C

Storage Comment: BMP-4 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.

Target Details for BMP4

Target: BMP4 (Bone Morphogenetic Protein 4 (BMP4))

Alternative Name: BMP-4

Background: BMPs (bone morphogenetic proteins) belong to the TGF beta superfamily of structurally related signaling proteins. Members of this superfamily are widely represented throughout the animal kingdom and have been implicated in a variety of developmental processes. Proteins of the TGF beta superfamily are disulfide-linked dimers composed of two 12-15 kDa polypeptide chains. As implied by their name, BMPs initiate, promote and regulate bone development, growth, remodeling and repair. Smad1 translocation to the nucleus is observed after the addition of BMP4 (also designated BMP2B), suggesting that BMP4 may play a role in activation of the Smad pathway. BMP is secreted into the extracellular matrix.

Gene ID: 652

UniProt: P12644

Pathways: Steroid Hormone Mediated Signaling Pathway, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development