Connexin antibody (C-Term)
The Rabbit Polyclonal anti-Connexin antibody has been validated for WB. It is suitable to detect Connexin in samples from Chicken, Dog, Human, Mouse, Rat, Sheep, Xenopus laevis and Zebrafish (Danio rerio).
Catalog No. ABIN5688865
Quick Overview for Connexin antibody (C-Term) (ABIN5688865)
Target
Connexin
Reactivity
Chicken, Dog, Human, Mouse, Rat, Sheep, Xenopus laevis, Zebrafish (Danio rerio)
Host
Rabbit
Clonality
Polyclonal
Application
Western Blotting (WB)
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Binding Specificity
- C-Term
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Purification
- Affinity Purified
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Immunogen
- Peptide corresponding to amino acid residues from the C-terminal region of rat connexin43.
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Application Notes
- The antibody has been directly tested for reactivity in Western blots in rat tissues. It is anticipated that the antibody will also work with chicken, dog, finch, human, mouse, non-human primate, sheep, Xenopus and zebra fish tissues based on the fact that these species have 100 % homology with the amino acid sequence used as antigen.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- 100 μLin 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.
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Storage
- -20 °C
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Storage Comment
- Connexin antibody can be stored at -20°C and is stable at -20°C for at least 1 year.
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- Connexin
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Background
- Gap junctional intercellular communication is thought to play a key role in development and may also be involved in epilepsy (Aronica et al., 2001). Connexin43 forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Permeability of connexin43 is known to be regulated by phosphorylation at er368 by protein kinase C (Yogo et al., 2002, Bao et al., 2004a). Phosphorylation of Ser368 by PKC induces a conformational change of connexin43 that results in a decrease in gap junction permeability (Bao et al., 2004b).
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Molecular Weight
- 43 kDa
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Gene ID
- 24392
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UniProt
- P08050
Target
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