AQP2 antibody (pSer261)

Catalog No. ABIN5688893

This anti-AQP2 antibody is a Rabbit Polyclonal antibody detecting AQP2 in WB and IHC. Suitable for Human, Rat, Mouse, Cow, Dog and Chicken. This Primary Antibody has been cited in 1 publication.

Quick Overview for AQP2 antibody (pSer261) (ABIN5688893)

Target: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Reactivity: Human, Rat, Mouse, Cow, Dog, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AQP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-AQP2 Antibody

Binding Specificity: pSer261

Purification: Affinity Purified

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser261 of rat aquaporin 2.

Application Details

Application Notes: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with canine, bovine, chicken, human, mouse and non-human primate tissues based on the fact that these species have 100 % homology with the amino acid sequence used as antigen.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μLin 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: Aquaporin 2 antibody can be stored at -20°C and is stable at -20°C for at least 1 year.

References for anti-AQP2 antibody (ABIN5688893)

Sugito, Etriwati, Akmal, Rahmi, Delima, Muchlisin, Hasan: "Immunohistochemical Expression of AQP2 and HSP70 in Broiler Kidney Tissue Treated with Salix tetrasperma Roxb. Extract under Heat Exposure." in: TheScientificWorldJournal, Vol. 2021, pp. 8711286, (2022) (PubMed).

Target Details for AQP2

Target: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Alternative Name: Aquaporin 2

Background: Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans (Iolascon et al.,2007). A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells (van Balkom et al., 2002). Recently, serine-261 has been identified as a novel phosphorylation site on AQP2 and levels of phosphorylated S261 have been shown to decrease with vasopressin treatment suggesting its involvement in vasopressin-dependent AQP2 trafficking (Hoffert et al., 2007).

Molecular Weight: 29/37 kDa

Gene ID: 25386

UniProt: P34080

Pathways: Response to Water Deprivation