ERK1/2 antibody (pThr202, pTyr204)

Catalog No. ABIN5688926

This anti-ERK1/2 antibody is a Rabbit Polyclonal antibody detecting ERK1/2 in WB and IHC. Suitable for Human, Mouse, Rat and Xenopus laevis.

Quick Overview for ERK1/2 antibody (pThr202, pTyr204) (ABIN5688926)

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Reactivity: Human, Mouse, Rat, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERK1/2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ERK1/2 Antibody

Binding Specificity: pThr202, pTyr204

Specificity: ERK1/2 antibody is specific for the 42/44 k ERK/MAPK protein phosphorylated at Thr202 and Tyr204..

Purification: Affinity Purified

Immunogen: ERK/MAPK (Thr202/Tyr204) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Thr202 and Tyr204 of ERK/MAPK.

Application Details

Application Notes: Immunolabeling in UV treated Jurkat Cells or in a rat brain lysate is blocked by the Thr202/Tyr204 phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Applications include Dot Blots (DB) and Western Blots (WB). Human, mouse, rat and Xenopus have 100 % amino acid sequence identity with the antigen used to raise the antibody. Dot blots and Western blots with a rat brain lysate were performed with each lot. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μLin 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20°C.

Target Details for ERK1/2

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Alternative Name: ERK1 / 2

Background: Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory. The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204.

Molecular Weight: 42/44 kDa

Gene ID: 116590

UniProt: P63086