FGF17 antibody

Catalog No. ABIN5688989

This anti-FGF17 antibody is a Rabbit Polyclonal antibody detecting FGF17 in WB, ELISA and Neut. Suitable for Human.

Quick Overview for FGF17 antibody (ABIN5688989)

Target: FGF17 (Fibroblast Growth Factor 17 (FGF17))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FGF17 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-FGF17 Antibody

Purification: Anti-hFGF-17 specific antibody was purified by affinity chromatography employing immobilized hFGF-17 matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFGF-17 (human Fibroblast Growth Factor-17).

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hFGF-17 (1.5 ng/mL), a concentration of 0.02 - 0.035 μ,g/mL of this antibody is required.

ELISA:

To detect hFGF-17 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hFGF-17.

Sandwich: To detect hFGF-17 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human FGF-17 (XP-5133Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-17.

Western Blot:
To detect hFGF-17 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-17 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: FGF-17 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for FGF17

Target: FGF17 (Fibroblast Growth Factor 17 (FGF17))

Alternative Name: FGF-17

Gene ID: 8822

UniProt: O60258

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway