CCL4 antibody

Catalog No. ABIN5689081

The Rabbit Polyclonal anti-CCL4 antibody has been validated for WB, ELISA and Neut. It is suitable to detect CCL4 in samples from Human.

Quick Overview for CCL4 antibody (ABIN5689081)

Target: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CCL4 Antibody

Purification: Anti-hMIP-1-beta specific antibody was purified by affinity chromatography employing immobilized hMIP-1-beta matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMIP-1-beta.

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-1-beta (50.00 ng/mL), a concentration of 1.75 - 3.0 μ,g/mL of this antibody is required.

ELISA:

To detect hMIP-1-beta by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMIP-1-beta.

Sandwich:

To detect hMIP-1β, by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human MIP-1β, (XP-5235Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-1β,.

Western Blot:
To detect hMIP-1-beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-1-beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: MIP-1B antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for CCL4

Target: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Alternative Name: MIP-1B

Gene ID: 6351

UniProt: P13236