CCL19 antibody

Catalog No. ABIN5689087

The Rabbit Polyclonal anti-CCL19 antibody has been validated for WB, ELISA and Neut. It is suitable to detect CCL19 in samples from Human.

Quick Overview for CCL19 antibody (ABIN5689087)

Target: CCL19 (Chemokine (C-C Motif) Ligand 19 (CCL19))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL19 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CCL19 Antibody

Purification: Anti-hMIP-3β, specific antibody was purified by affinity chromatography employing immobilized hMIP-3β, matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMIP-3β, (human Macrophage Inflammatory Protein-3 beta).

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-3β, (100 ng/mL), a concentration of 2.7 4.0 μ,g/mL of this antibody is required.

ELISA:

To detect hMIP-3β, by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMIP-3β,.

Sandwich:

To detect hMIP-3β, by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human MIP-3β, (XP-5241Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-3β,.

Western Blot:
To detect hMIP-3β, by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-3β, is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: MIP-3B antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for CCL19

Target: CCL19 (Chemokine (C-C Motif) Ligand 19 (CCL19))

Alternative Name: MIP-3B

Gene ID: 6364

UniProt: P78556

Pathways: Positive Regulation of Immune Effector Process