CCL25 antibody

Catalog No. ABIN5689117

The Rabbit Polyclonal anti-CCL25 antibody has been validated for ELISA, WB and Neut. It is suitable to detect CCL25 in samples from Human.

Quick Overview for CCL25 antibody (ABIN5689117)

Target: CCL25 (Chemokine (C-C Motif) Ligand 25 (CCL25))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL25 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Neutralization (Neut)

Product Details anti-CCL25 Antibody

Purification: Anti-hTECK specific antibody was purified by affinity chromatography employing immobilized hTECK matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTECK (human Thymus Expressed Chemokine).

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hTECK (100 ng/mL), a concentration of 10.0- 15.0 μ,g/mL of this antibody is required.

ELISA:

To detect hTECK by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hTECK.

Sandwich:

To detect hTECK by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human TECK (XP-5280Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTECK.

Western Blot:
To detect hTECK by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTECK is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: TECK antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for CCL25

Target: CCL25 (Chemokine (C-C Motif) Ligand 25 (CCL25))

Alternative Name: TECK

Background: This gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. TECK displays chemotactic activity for dendritic cells, thymocytes, and activated macrophages but is inactive on peripheral blood lymphocytes and neutrophils. TECK binds to chemokine receptor CCR9. Two alternatively spliced variants encoding two different isoforms have been identified.

Gene ID: 6370

UniProt: O15444