CXCL10 antibody

Catalog No. ABIN5689154

This anti-CXCL10 antibody is a Rabbit Polyclonal antibody detecting CXCL10 in WB, ELISA and Neut. Suitable for Mouse.

Quick Overview for CXCL10 antibody (ABIN5689154)

Target: CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL10 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CXCL10 Antibody

Purification: Anti-mIP-10 specific antibody was purified by affinity chromatography employing immobilized mIP-10 matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIP-10 (murine IP-10).

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mIP-10 (15.00 ng/mL), a concentration of 0.5 0.9 μ,g/mL of this antibody is required.

ELISA:

To detect mIP-10 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIP-10.

Sandwich:
br>To detect mIP-10 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine IP-10 (XP-5204Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIP-10.

Western Blot:

To detect mIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ng/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIP-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: IP-10 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for CXCL10

Target: CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))

Alternative Name: IP-10

Background: Interferon-gamma-inducible 10 kD protein (IP-10), is a CXC chemokine with chemoattractant properties for CD4-positive T cells and inhibits early normal and leukemic hemopoietic progenitor proliferation. IP-10 is produced by a wide variety of cell types ranging from neutrophils and monocytes to hepatocytes, endothelial cells and keratinocytes. The cytokine is reported to be involved in a scala of inflammatory pathologies such as HIV encephalitis, cutaneous T cell lymphoma, chronic hepatitis and acute anterior uveitis. Various observations strongly suggest a role for the CXC chemokines IL-8 and IP-10 in the regulation of angiogenic activity in cancer and in idiopathic pulmonary fibrosis.

Gene ID: 15945

UniProt: P17515