CXCL2 antibody

Catalog No. ABIN5689169

This anti-CXCL2 antibody is a Rabbit Polyclonal antibody detecting CXCL2 in WB, ELISA and Neut. Suitable for Mouse.

Quick Overview for CXCL2 antibody (ABIN5689169)

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CXCL2 Antibody

Purification: Anti-mMIP-2 specific antibody was purified by affinity chromatography employing immobilized mMIP-2 matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mMIP-2 (murine Macrophage Inflammatory Protein-2).

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mMIP-2 (15 ng/mL), a concentration of 0.52 - 0.9 μ,g/mL of this antibody is required.

ELISA:

To detect mMIP-2 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mMIP-2.

Sandwich:

To detect mMIP-2 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine MIP-2 (XP-5237Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMIP-2.

Western Blot:
To detect mMIP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMIP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: MIP-2 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for CXCL2

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Alternative Name: MIP-2

Gene ID: 20310

UniProt: P10889

Pathways: Cellular Response to Molecule of Bacterial Origin