CXCL2 antibody (Biotin)

Catalog No. ABIN5689182

This Rabbit Polyclonal antibody specifically detects CXCL2 in WB and ELISA. It exhibits reactivity toward Rat.

Quick Overview for CXCL2 antibody (Biotin) (ABIN5689182)

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL2 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-CXCL2 Antibody (Biotin)

Purification: Anti-rat GRO/β,/MIP-2 specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant rat GRO/β,/MIP-2 (rat GRO/β,/MIP-2).

Application Details

Application Notes: ELISA:

To detect rat GRO/β,/MIP-2 by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant rat GRO/β,/MIP-2.

Sandwich: To detect Rat GRO-β,/MIP-2 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Rat GRO-β,/MIP-2 (XP-5149) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat GRO-β,/MIP-2.

Western Blot:

To detect rat GRO/β,/MIP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat GRO/β,/MIP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: GROB/MIP-2 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for CXCL2

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Alternative Name: MIP-2

Background: Macrophage inflammatory protein 2 (MIP-2) is a chemokine that attracts neutrophils, and its secretion from intestinal epithelial cells is enhanced by inflammatory stimuli such as interleukin 1beta.

Gene ID: 114105

UniProt: P30348

Pathways: Cellular Response to Molecule of Bacterial Origin