CXCL2 antibody

Catalog No. ABIN5689185

This Rabbit Polyclonal antibody specifically detects CXCL2 in WB, ELISA and Neut. It exhibits reactivity toward Rat.

Quick Overview for CXCL2 antibody (ABIN5689185)

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CXCL2 Antibody

Purification: Anti-Rat GROβ,/MIP-2 specific antibody was purified by affinity chromatography employing immobilized Rat GROβ,/MIP-2 matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat GROβ,/MIP-2 (Rat GROβ,/Macrophage Inflammatory Protein-2).

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Rat GROβ,/MIP-2 (100 ng/mL), a concentration of 3.0 - 5.0 μ,g/mL of this antibody is required.

ELISA:

To detect Rat GROβ,/MIP-2 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant Rat GROβ,/MIP-2.

Sandwich: To detect Rat GROβ,/MIP-2 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat GROβ,/MIP-2 (XP-5149Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat GROβ,/MIP-2.

Western Blot:

To detect Rat GROβ,/MIP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat GROβ,/MIP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: GROB/MIP-2 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for CXCL2

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Alternative Name: MIP-2

Background: Macrophage inflammatory protein 2 (MIP-2) is a chemokine that attracts neutrophils, and its secretion from intestinal epithelial cells is enhanced by inflammatory stimuli such as interleukin 1beta.

Gene ID: 114105

UniProt: P30348

Pathways: Cellular Response to Molecule of Bacterial Origin