CCL5 antibody (Biotin)

Catalog No. ABIN5689197

This Rabbit Polyclonal antibody specifically detects CCL5 in WB and ELISA. It exhibits reactivity toward Rat.

Quick Overview for CCL5 antibody (Biotin) (ABIN5689197)

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL5 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-CCL5 Antibody (Biotin)

Purification: Anti-rat RANTES specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant rat RANTES (rat RANTES).

Application Details

Application Notes: ELISA:

To detect rat RANTES by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant rat RANTES.

Sandwich:

To detect Rat RANTES by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Rat RANTES (XP-5258) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat RANTES.

Western Blot:

To detect rat RANTES by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat RANTES is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: RANTES antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for CCL5

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Alternative Name: RANTES

Background: RANTES is a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. It binds to CCR1, CCR3, CCR4 and CCR5 and is one of the major HIV suppressive factors produced by CD8+ T cells. Recombinant RANTES protein induces a dose dependent inhibition of different strains of HIV 1, HIV 2, and simian immunodeficiency virus (SIV). It is T cell and macrophage specific, induced by mitogens, and belongs to the intercrine beta (chemokine CC) family.

Gene ID: 81780

UniProt: P50231

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration