alpha Adducin antibody (AA 724-728)

Catalog No. ABIN5689375

The Rabbit Polyclonal anti-alpha Adducin antibody has been validated for WB, IHC and IF. It is suitable to detect alpha Adducin in samples from Human, Mouse and Rat.

Quick Overview for alpha Adducin antibody (AA 724-728) (ABIN5689375)

Target: alpha Adducin (ADD1) (Adducin 1 (Alpha) (ADD1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Adducin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-alpha Adducin Antibody

Binding Specificity: AA 724-728

Specificity: This antibody detects endogenous level of total ADD1 protein.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: ADD1 (Ab-726) antibody was raised against a peptide sequence around aa.724~728 (T-P-S-F-L) derived from Human ADD1.

Application Details

Application Notes: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for alpha Adducin

Target: alpha Adducin (ADD1) (Adducin 1 (Alpha) (ADD1))

Alternative Name: ADD1

Background: Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms, however, not all variants have been fully described.

Molecular Weight: 130 kDa

Gene ID: 118

NCBI Accession: NP_001110

UniProt: P35611

Pathways: Negative Regulation of Hormone Secretion, Regulation of Actin Filament Polymerization, Regulation of Lipid Metabolism by PPARalpha, ER-Nucleus Signaling