MARCKS antibody (pSer162)

Catalog No. ABIN5689645

The Rabbit Polyclonal anti-MARCKS antibody has been validated for WB and IF. It is suitable to detect MARCKS in samples from Human, Mouse and Rat.

Quick Overview for MARCKS antibody (pSer162) (ABIN5689645)

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARCKS antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-MARCKS Antibody

Binding Specificity: pSer162

Specificity: This antibody detects endogenous level of MARCKS only when phosphorylated at serine 162.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Immunogen: MARCKS (Phospho-Ser162) antibody was raised against a peptide sequence around phosphorylation site of serine 162 (K-K-S (p) -F-K) derived from Human MARCKS.

Application Details

Application Notes: Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for MARCKS

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Alternative Name: MARCKS

Background: MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.

Molecular Weight: 80 kDa

Gene ID: 4082

NCBI Accession: NP_002347

UniProt: P29966