CTGF antibody

Catalog No. ABIN5689933

The Rabbit Polyclonal anti-CTGF antibody has been validated for WB, ELISA and Neut. It is suitable to detect CTGF in samples from Human.

Quick Overview for CTGF antibody (ABIN5689933)

Target: CTGF (Connective Tissue Growth Factor (CTGF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CTGF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CTGF Antibody

Purification: CTGF antibody was purified by affinity chromatography employing immobilized CTGF matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hCTGF. Human CTGF specific antibody was purified by affinity chromatography employing immobilized hCTGF matrix.

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hCTGF (5.0 μ,g/mL), a concentration of 6.0 - 9.0 μ,g/mL of this antibody is required.

ELISA:
Indirect:
To detect hCTGF by indirect ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCTGF.
Sandwich:
To detect hCTGF by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required.

Western Blot:
To detect hCTGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCTGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: CTGF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for CTGF

Target: CTGF (Connective Tissue Growth Factor (CTGF))

Alternative Name: CTGF

Background: CTGF (connective tissue growth factor) is a 38  kDa, cysteine-rich, secreted peptide. It is a new member of the peptide family that include serum-induced immediate early gene products, a v-src-induced peptide and a putative proto-oncogene. Among the many functions of the CTGF gene family are embryogenesis, wound healing and regulation of extracellular matrix production.

Gene ID: 1490

UniProt: P29279

Pathways: Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Growth Factor Binding