RETNLB antibody (Biotin)

Catalog No. ABIN5690223

This Rabbit Polyclonal antibody specifically detects RETNLB in WB and ELISA. It exhibits reactivity toward Mouse.

Quick Overview for RETNLB antibody (Biotin) (ABIN5690223)

Target: RETNLB (Resistin Like beta (RETNLB))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RETNLB antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-RETNLB Antibody (Biotin)

Purification: RELMb specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mRELMβ,. Murine RELMβ, specific antibody was purified by affinity chromatography and then biotinylated.

Application Details

Application Notes: ELISA:

Direct:
To detect mRELMb by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mRELMb.
Sandwich:
To detect mRELMb by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required.

Western Blot:
To detect mRELMb by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mRELMb is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: RELMb antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for RETNLB

Target: RETNLB (Resistin Like beta (RETNLB))

Alternative Name: RELMb

Background: RELM beta, is a secreted protein, a probable hormone, expressed only in the gastrointestinal tract, particularly the colon. The biological functions of RELM beta and its molecular targets, are not fully known but, it has been suggested that it plays a regulatory role during inflammation and may also act to establish links among adipose tissue, the intestine and the liver. Interestingly the molecular structure of RELM beta is highly homologous to that of the adipose-derived cytokine Resistin and RELM alpha.

Gene ID: 57263

UniProt: Q99P86

Pathways: Hormone Activity