RHOT1 antibody (AA 40-90)

Catalog No. ABIN5690226

This Rabbit Polyclonal antibody specifically detects RHOT1 in WB, ELISA, IF and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RHOT1 antibody (AA 40-90) (ABIN5690226)

Target: RHOT1 (Ras Homolog Gene Family, Member T1 (RHOT1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RHOT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RHOT1 Antibody

Binding Specificity: AA 40-90

Specificity: RHOT1 antibody is human, mouse and rat reactive. At least three isoforms of RHOT1 are known to exist, this antibody will detect all three isoforms.

Purification: RHOT1 antibody is affinity chromatography purified via peptide column.

Immunogen: RHOT1 antibody was raised against a 15 amino acid peptide near the amino terminus of human RHOT1.

The immunogen is located within amino acids 40 - 90 of RHOT1.

Isotype: IgG

Application Details

Application Notes: RHOT1 antibody can be used for detection of RHOT1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: RHOT1 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: RHOT1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for RHOT1

Target: RHOT1 (Ras Homolog Gene Family, Member T1 (RHOT1))

Alternative Name: RHOT1

Background: The Ras homolog family member T1 (RHOT) is an atypical Rho Ca2+-binding GTPase that localizes to the mitochondria (1). RHOT1, the related protein RHOT2, the adaptor protein Milton, and the PTEN induced putative kinase 1 (PINK1), form a complex that is involved in axonal transport of mitochondria (2,3). Both PINK1 and Parkin target RHOT1 for phosphorylation and degradation, causing the arrest of mitochondrial motility (4).

Molecular Weight: Predicted: 76 kDa

Observed: 78 kDa

Gene ID: 55288

NCBI Accession: NP_001028740

UniProt: Q8IXI2