CCL28 antibody (Biotin)

Catalog No. ABIN5690479

The Rabbit Polyclonal anti-CCL28 antibody has been validated for WB and ELISA. It is suitable to detect CCL28 in samples from Human.

Quick Overview for CCL28 antibody (Biotin) (ABIN5690479)

Target: CCL28 (Chemokine (C-C Motif) Ligand 28 (CCL28))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL28 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-CCL28 Antibody (Biotin)

Immunogen: Produced from sera of rabbits immunized with highly pure recombinant Human MEC. Human MEC specific antibody was purified by affinity chromatography and then biotinylated.

Application Details

Application Notes: ELISA:
Direct:
To detect hMEC by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMEC. Sandwich:To detect hMEC by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci's Polyclonal Anti-Human MEC (38-268) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMEC.

Western Blot:

To detect hMEC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMEC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for CCL28

Target: CCL28 (Chemokine (C-C Motif) Ligand 28 (CCL28))

Alternative Name: MEC

Gene ID: 56477

UniProt: Q9NRJ3