CCL27 antibody (Biotin)

Catalog No. ABIN5690483

This anti-CCL27 antibody is a Rabbit Polyclonal antibody detecting CCL27 in WB and ELISA. Suitable for Human.

Quick Overview for CCL27 antibody (Biotin) (ABIN5690483)

Target: CCL27 (Chemokine (C-C Motif) Ligand 27 (CCL27))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL27 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-CCL27 Antibody (Biotin)

Immunogen: Produced from sera of rabbits immunized with highly pure recombinant Human CTACK. Human CTACK specific antibody was purified by affinity chromatography and then biotinylated.

Application Details

Application Notes: ELISA:
Direct:
To detect Human CTACK by direct ELISA (using 100 μ,L/well) a concentration of approximately 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human CTACK.

Sandwich
To detect Human CTACK by sandwich ELISA (using 100 μ,L/well) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci's Polyclonal Anti-Human CTACK (38-263) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human CTACK.

Western Blot:

To detect Human CTACK by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. When used in conjunction with compatible development reagents the detection limit for recombinant Human CTACK is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for CCL27

Target: CCL27 (Chemokine (C-C Motif) Ligand 27 (CCL27))

Alternative Name: CTACK

Gene ID: 10850

UniProt: Q5VZ77