CXCL10 antibody

Catalog No. ABIN5690512

The Rabbit Polyclonal anti-CXCL10 antibody has been validated for WB, ELISA and Neut. It is suitable to detect CXCL10 in samples from Rat.

Quick Overview for CXCL10 antibody (ABIN5690512)

Target: CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL10 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CXCL10 Antibody

Immunogen: Produced from sera of rabbits immunized with highly pure recombinant Human Heregulin- β,1. Human Heregulin- β,1 specific antibody was purified by affinity chromatography and then biotinylated.

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Rat IP-10 (100 ng/mL), a concentration of 5.0-10.0 μ,g/mL of this antibody is required.

Sandwich
To detect Rat IP-10 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat IP-10 (38-255) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IP-10.

Western Blot:
To detect Rat IP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IP-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for CXCL10

Target: CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))

Alternative Name: IP-10

Gene ID: 245920

UniProt: P48973