FASL antibody (AA 196-220)

Catalog No. ABIN5690653

The Rat Monoclonal anti-FASL antibody has been validated for FACS and IHC. It is suitable to detect FASL in samples from Mouse.

Quick Overview for FASL antibody (AA 196-220) (ABIN5690653)

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This FASL antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC)

Clone: A11

Product Details anti-FASL Antibody

Binding Specificity: AA 196-220

Purification: FasL (monoclonal A11) antibody was purified to ≥,95% in SDS-PAGE.

Immunogen: FasL (monoclonal A11) antibody was raised against a synthetic peptide corresponding to amino acids 196-220 of mouse FasL.

Isotype: IgM

Application Details

Application Notes: FasL antibody can be used for the detection of mouse FasL by flow cytometry and immunohistochemistry (frozen sections and paraffin sections).Method: Cryostat sections (6-8 μ,m) were air dried, fixed in 4 % paraformaldehyde in PBS and used immediately. Sections were incubated for 1 hr at room temperature (RT) with 20 μ,g/mL of A11 antibody in PBS, 0.2 % BSA, 0.05 % Tween 20. After rinsing in PBS, a FITC-conjugated anti-rat IgM was added for 30 min., slides washed again in PBS and visualized using a fluorescence microscope.Paraffin sections: Dewax in xylene (2x10 min), EtOH 100 % (2x10 min), block endogeneous peroxidase (0.6 % H2O2 in Methanol 10 min), rehydrate through graded EtOH (100 %, 95 %, 70 %), rinse in H20, unmask epitopes by heating slides in citrate buffer (0.01M,  pH 6.0) 2x5 min in microwave oven (650-750W), cool down to RT 20 min, wash 1x in PBS, incubate with A11 20 μ,g/mL antibody, wash in PBS, incubate in anti-rat IgM HRP, wash in PBS, develop in appropriate substrate solution (DAB or AEC).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: FasL antibody can be stored at 4°C, stable for one year.

Target Details for FASL

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Alternative Name: FasL

Gene ID: 14103

UniProt: P41047

Pathways: Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity