ITGAL antibody

Catalog No. ABIN5691085

The Mouse Monoclonal anti-ITGAL antibody has been validated for FACS, IP and ICC. It is suitable to detect ITGAL in samples from Human.

Quick Overview for ITGAL antibody (ABIN5691085)

Target: ITGAL (Integrin alpha-L (ITGAL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGAL antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: NKI (SPV)-L1

Product Details anti-ITGAL Antibody

Specificity: The antibody NKI (SPV)-L1 is directed against human CD11a. The antibody is reported to inhibit the cytolytic activity of various human cytotoxic T-cell clones including HG-38 and HG-31

Immunogen: NKI (SPV)-L1 is a mouse monoclonal IgG1 antibody derived by fusion of Sp2/0 mouse myeloma cells with splenocytes from a BALB/c mouse immunized with cytotoxic T-cell clone HG-38.

Isotype: IgG1

Application Details

Application Notes: The NKI (SPV)-L1 antibody is suitable for the detection of CD11a by immunoprecipitation, immunocytochemistry, immunohistochemistry on frozen sections and by flow cytometry. Human tonsil is suitable for use as a positive control tissue. Optimal antibody dilutions for the different applications should be determined by titration.

Restrictions: For Research Use only

Handling

Buffer: Each vial contains 100 μL 1 mg/mL purified monoclonal antibody in phosphate buffered saline (PBS) containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C, or in small aliquots at -20°C.

Target Details for ITGAL

Target: ITGAL (Integrin alpha-L (ITGAL))

Alternative Name: CD11a, Integrin alpha-L

Background: Integrin alpha L (antigen CD11A, p180, lymphocyte function-associated antigen 1, alpha polypeptide), also known as ITGAL, is a human gene which functions in the immune system. It is involved in cellular adhesion and co-stimulatory signaling. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 is part of the family of leukocyte integrins that are recognised by their common β-chains (CD18) and the distinct α-chain CD11a. LFA-1 is expressed on lymphocytes, monocytes and granulocytes. It is involved in recruitment of these cells to the site of infection where it binds to ICAM-1 on antigen-presenting cells and functions as an adhesion molecule. LFA-1 is the first to bind T-cells to antigen-presenting cells and initially binds weakly. A signal from the T-cell receptor and/or the cytokine receptor changes the conformation and prolongs the cell contact, allowing the T-cell to proliferate. Blocking experiments with anti-LFA monoclonal antibodies demonstrated that LFA-1 inhibits the adhesion step between effector and target cells in cytotoxic T lymphocyte, natural killer and lectin dependent cellular cytotoxicity. LFA-1 also acts as an adhesion molecule between monocytes and T-cells.

UniProt: P20701

Pathways: Activated T Cell Proliferation, Integrin Complex