CD90 antibody

Catalog No. ABIN5691126

This Rabbit Polyclonal antibody specifically detects CD90 in ICC and IHC (fro). It exhibits reactivity toward Mouse and Rat.

Quick Overview for CD90 antibody (ABIN5691126)

Target: CD90 (THY1)

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD90 antibody is un-conjugated

Application: Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CD90 Antibody

Specificity: Thy-1 antiserum is directed against rat thymocyte-1 antigen (Thy-1) and also reacts with mouse Thy-1.

Immunogen: Thy-1 antibody is a rabbit polyclonal antiserum obtained from rabbits immunised with purified Thy-1 derived from rat brain.

Application Details

Application Notes: Thy-1 is suitable for the detection of rat and mouse Thy-1 by immunohistochemical staining of frozen tissue sections. After drying, the sections can be either left unfixed or fixed with acetone at 4 °C for 4 min or 95 % ethanol at 4 °C for 15 min or 4 % formaldehyde in phosphate buffered saline pH 7.4 at 20 °C for 15 min. Also isolated cells can be immunocytochemically stained by this antiserum. Optimal antibody dilutions for the different applications should be determined by titration. Recommended range 1:50 - 1:100.

Restrictions: For Research Use only

Handling

Buffer: Each vial contains 100 μL polyclonal antiserum containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C, or in small aliquots at -20°C.

Target Details for CD90

Target: CD90 (THY1)

Alternative Name: Thy-1/CD90

Background: Thymocyte-1 antigen (Thy-1), also known as CD90, is a glycophosphatidylinositol (GPI)-anchored cell surface glycoprotein that is a member of the immunoglobulin superfamily. Expression of Thy-1, originally identified as a marker of thymocyte differentiation in mice, has been found to vary between species. In rats, unlike mice, Thy-1 is expressed on bone marrow cells but not on peripheral T-cells. In both mice and rats, Thy-1 is abundantly expressed on thymocytes and neuronal cells and to a lesser extent on fibroblasts, myofibroblasts, vascular endothelial cells, mammary glands and other non-lymphoid tissues. In many tissues the level of Thy-1 expression changes during cell differentiation. The exact function of Thy-1 has not been fully determined but studies suggest that Thy-1 may be an important regulator of cell-cell and cell-matrix interactions due to its involvement in a range of biological processes including cell growth and differentiation, cell adhesion and migration, apoptotic signalling and tumour suppression.

UniProt: P01830, P01831

Pathways: Cell-Cell Junction Organization