ZAK antibody (AA 276-511)

Catalog No. ABIN5692782

This Rabbit Polyclonal antibody specifically detects ZAK in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ZAK antibody (AA 276-511) (ABIN5692782)

Target: ZAK (Mitogen-Activated Protein Kinase Kinase Kinase MLT (ZAK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZAK antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ZAK Antibody

Binding Specificity: AA 276-511

Purpose: Anti-MAP3K20 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MAP3K20 Antibody Picoband® (ABIN5692782). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E. coli-derived human MAP3K20 recombinant protein (Position: F276-E511).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. "Entrez Gene: ZAK sterile alpha motif and leucine zipper containing kinase AZK". 2. Yang, Jaw-Ji (Jan 2003). "A novel zinc finger protein, ZZaPK, interacts with ZAK and stimulates the ZAK-expressing cells re-entering the cell cycle". Biochem. Biophys. Res. Commun. United States. 301 (1): 71-7.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ZAK

Target: ZAK (Mitogen-Activated Protein Kinase Kinase Kinase MLT (ZAK))

Alternative Name: MAP3K20

Background:

Synonyms: Mitogen-activated protein kinase kinase kinase 20

Tissue Specificity: Ubiquitously expressed. Isoform 2 is the predominant form in all tissues examined, except for liver, in which isoform 1 is more highly expressed.

Background: Sterile alpha motif and leucine zipper containing kinase AZK, also known as ZAK, is a human gene. This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Molecular Weight: 91 kDa

Gene ID: 51776