Laminin gamma 1 antibody (all Isoforms)

Catalog No. ABIN5692889

This anti-Laminin gamma 1 antibody is a Rabbit Polyclonal antibody detecting Laminin gamma 1 in WB, IHC and IF. Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 2+ publications.

Quick Overview for Laminin gamma 1 antibody (all Isoforms) (ABIN5692889)

Target: Laminin gamma 1 (LAMC1) (Laminin, gamma 1 (LAMC1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Laminin gamma 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Laminin gamma 1 Antibody

Binding Specificity: all Isoforms

Purpose: Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody Picoband®

Sequence: NKLNEIEGSL NKAKDEMKAS ,DLEERVRRQR NHLHLLETSI ,LQLDSHGALH HKLRQLEEES

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody Picoband® (ABIN5692889). Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: Peptide mixture of laminin gamma1,2,3 (NKLNEIEGSLNKAKDEMKAS, DLEERVRRQRNHLHLLETSI, LQLDSHGALHHKLRQLEEES). Laminin gamma has only three subtypes of antibody to gamma1-3 reactive with all isoforms of laminin.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat,
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat
Immunofluorescence, 2 μg/mL, Mouse, Rat
1. Aumailley M et al. (2005). "A simplified laminin nomenclature". Matrix Biol. 24 (5): 326-32. 2. Colognato H, Yurchenco P (2000). "Form and function: the laminin family of heterotrimers".Dev. Dyn. 218 (2): 213-34. 3. Timpl R et al. (1979). "Laminin - a glycoprotein from basement membranes". J Biol Chem 254 (19): 9933-7.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-Laminin gamma 1 antibody (ABIN5692889)

Fu, Hu, Dang, Chang, Du, Wu, Gao: "Remarkable preservation of Ca(2+) homeostasis and inhibition of apoptosis contribute to anti-muscle atrophy effect in hibernating Daurian ground squirrels." in: Scientific reports, Vol. 6, pp. 27020, (2018) (PubMed).

Chen, Wang, Liu, Fang, Tan, Zhou, Zhou, Lv: "Highly effective ex vivo gene manipulation to study kidney development using self-complementary adenoassociated viruses." in: TheScientificWorldJournal, Vol. 2014, pp. 682189, (2014) (PubMed).

Target Details for Laminin gamma 1

Target: Laminin gamma 1 (LAMC1) (Laminin, gamma 1 (LAMC1))

Alternative Name: Lamc1

Background:

Synonyms: Laminin subunit gamma-1,Laminin B2 chain,Laminin-1 subunit gamma,Laminin-10 subunit gamma,Laminin-11 subunit gamma,Laminin-2 subunit gamma,Laminin-3 subunit gamma,Laminin-4 subunit gamma,Laminin-6 subunit gamma,Laminin-7 subunit gamma,Laminin-8 subunit gamma,Laminin-9 subunit gamma,S-laminin subunit gamma,S-LAM gamma,Lamc1,Lamb-2, Lamc-1,

Tissue Specificity: Found in the basement membranes (major component).

Background: Tumor necrosis factor ligand superfamily member 14 is a protein that in humans is encoded by the TNFSF14 gene. TNFSF14 has also been designated as CD258, as well as LIGHT. It was mapped on chromosome 19p13.3. The protein encoded by this gene is a member of the tumor necrosis factor(TNF) ligand family. This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. It has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported.

Molecular Weight: 150, 220-250 kDa

Gene ID: 226519

UniProt: P02468