TFF1 antibody (AA 22-87)

Catalog No. ABIN5692924

The Rabbit Polyclonal anti-TFF1 antibody has been validated for ELISA. It is suitable to detect TFF1 in samples from Mouse.

Quick Overview for TFF1 antibody (AA 22-87) (ABIN5692924)

Target: TFF1 (Trefoil Factor 1 (TFF1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TFF1 antibody is un-conjugated

Application: ELISA

Product Details anti-TFF1 Antibody

Binding Specificity: AA 22-87

Purpose: Anti-Tff1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Tff1 Antibody Picoband® (ABIN5692924). Tested in ELISA applications. This antibody reacts with Mouse.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived mouse Tff1 recombinant protein (Position: Q22-F87). Mouse Tff1 shares 67.2% and 83.6% amino acid (aa) sequence identity with human and rat Tff1, respectively.

Isotype: IgG

Application Details

Application Notes: ELISA Capture (Matched Antibody Pair), 2-8 μg/mL
1. Chutipongtanate, S., Nakagawa, Y., Sritippayawan, S., Pittayamateekul, J., Parichatikanond, P., Westley, B. R., May, F. E. B., Malasit, P., Thongboonkerd, V.Identification of human urinary trefoil factor 1 as a novel calcium oxalate crystal growth inhibitor.J. Clin. Invest. 115: 3613-3622, 2005. 2. Hanby, A. M., Poulsom, R., Singh, S., Elia, G., Jeffery, R. E., Wright, N. A.Spasmolytic polypeptide is a major antral peptide: distribution of the trefoil peptides human spasmolytic polypeptide and pS2 in the stomach.Gastroenterology 105: 1110-1116, 1993. 3. Roberts, M., Wallace, J., Jeltsch, J.-M., Berry, M.The 5-prime flanking region of the human pS2 gene mediates its transcriptional activation by estrogen in MCF-7 cells.Biochem. Biophys. Res. Commun. 151: 306-313, 1988.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for TFF1

Target: TFF1 (Trefoil Factor 1 (TFF1))

Alternative Name: Tff1

Background:

Synonyms: Trefoil factor 1,Protein pS2,Tff1,Bcei, Ps2,

Tissue Specificity: Strong expression found in normal gastric mucosa. Not detected in the small or large intestine nor in normal mammary gland.

Background: TFF1 (Trefoil factor 1), also known as pS2, is a protein that in humans is encoded by the TFF1 gene. Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. It is found that TFF1 in normal human urine inhibited the growth of calcium oxalate crystals. Urinary TFF1 showed an inhibitory potency similar to that of nephrocalcin, and inhibition was dose dependent and inhibited by TFF1 antisera, particularly by antisera ed to the TFF1 C terminus. Concentrations and relative amounts of TFF1 in the urine of patients with idiopathic calcium oxalate kidney stones were significantly less than those found in controls. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21.

Molecular Weight: 17 kDa

Gene ID: 21784

UniProt: Q08423

Pathways: EGFR Signaling Pathway