SP6 antibody

Catalog No. ABIN5692945

The Rabbit Polyclonal anti-SP6 antibody has been validated for WB, IHC, ICC and IF. It is suitable to detect SP6 in samples from Human, Mouse and Rat.

Quick Overview for SP6 antibody (ABIN5692945)

Target: SP6 (Sp6 Transcription Factor (SP6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SP6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-SP6 Antibody

Purpose: Anti-SP6 Antibody Picoband®

Sequence: QPDMSHHYES WFRPTHPGAE DGSWWDLHPG TSWMDLPH

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SP6 Antibody Picoband® (ABIN5692945). Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human SP6, which shares 97.4% amino acid (aa) sequence identity with both mouse and rat SP6.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry, 2-5 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
1. Nakamura, T., Unda, F., de-Vega, S., Vilaxa, A., Fukumoto, S., Yamada, K. M., Yamada, Y. The Kruppel-like factor epiprofin is expressed by epithelium of developing teeth, hair follicles, and limb buds and promotes cell proliferation. J. Biol. Chem. 279: 626-634, 2004. 2. Scohy, S., Gabant, P., Van Reeth, T., Hertveldt, V., Dreze, P.-L., Van Vooren, P., Riviere, M., Szpirer, J., Szpiper, C. Identification of KLF13 and KLF14 (SP6), novel members of the SP/XKLF transcription factor family. Genomics 70: 93-101, 2000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SP6

Target: SP6 (Sp6 Transcription Factor (SP6))

Alternative Name: SP6

Background:

Synonyms: Transcription factor Sp6, Krueppel-like factor 14, SP6, KLF14

Tissue Specificity: Ubiquitous.

Background: SP6 belongs to a family of transcription factors that contain 3 classical zinc finger DNA-binding domains consisting of a zinc atom tetrahedrally coordinated by 2 cysteines and 2 histidines (C2H2 motif). These transcription factors bind to GC-rich sequences and related GT and CACCC boxes. By somatic cell hybrid analysis and FISH, he SP6 gene is mapped t to chromosome 17q21.3-q22.

Molecular Weight: 40 kDa

Gene ID: 80320