alpha Fetoprotein antibody (AA 19-254)

Catalog No. ABIN5692976

This anti-alpha Fetoprotein antibody is a Rabbit Polyclonal antibody detecting alpha Fetoprotein in WB and ELISA. Suitable for Mouse and Rat. This Primary Antibody has been cited in 6+ publications.

Quick Overview for alpha Fetoprotein antibody (AA 19-254) (ABIN5692976)

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Fetoprotein antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-alpha Fetoprotein Antibody

Binding Specificity: AA 19-254

Purpose: Anti-Alpha 1 Fetoprotein/Afp Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Alpha 1 Fetoprotein/Afp Antibody Picoband® (ABIN5692976). Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E. coli-derived mouse Alpha 1 Fetoprotein recombinant protein (Position: K19-L254).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Bader D, Riskin A, Vafsi O, Tamir A, Peskin B, Israel N, Merksamer R, Dar H, David M (November 2004). "Alpha-fetoprotein in the early neonatal period--a large study and review of the literature". Clin. Chim. Acta 349 (1-2): 15-23. 2. Blair JI, Carachi R, Gupta R, Sim FG, McAllister EJ, Weston R (April 1987). "Plasma alpha fetoprotein reference ranges in infancy: effect of prematurity". Arch. Dis. Child. 62 (4): 362-9. 3. Carter CS (2002). "Neuroendocrinology of sexual behavior in the female". In Becker JB. Behavioral Endocrinology. Cambridge, Mass: MIT Press. pp.88-89. ISBN 0-262-52321-3

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-alpha Fetoprotein antibody (ABIN5692976)

Jeng, Jeng, Jeng, Sheen, Li, Lu, Chang: "Tropism of liver epithelial cells toward hepatocellular carcinoma in vitro and in vivo with altering gene expression of cancer stem cells." in: American journal of surgery, Vol. 215, Issue 4, pp. 735-743, (2017) (PubMed).

Winkler, Hempel, Brückner, Mallek, Weise, Liehr, Tautenhahn, Bartels, Christ: "Mouse white adipose tissue-derived mesenchymal stem cells gain pericentral and periportal hepatocyte features after differentiation in vitro, which are preserved in vivo after hepatic transplantation." in: Acta physiologica (Oxford, England), Vol. 215, Issue 2, pp. 89-104, (2016) (PubMed).

Yan, Zhu, Sun, Zhang, Li, Sun, Li, Qian, Zhu, Xu: "Extracellular regulated protein kinases 1/2 phosphorylation is required for hepatic differentiation of human umbilical cord-derived mesenchymal stem cells." in: Experimental biology and medicine (Maywood, N.J.), Vol. 240, Issue 4, pp. 534-45, (2015) (PubMed).

Shan, Wu, Li, Shen, Wang, Liu, Shen, Lei: "Continuous passages accelerate the reprogramming of mouse induced pluripotent stem cells." in: Cellular reprogramming, Vol. 16, Issue 1, pp. 77-83, (2014) (PubMed).

Wang, Xu, Zhou, Zhong, Wen, Yu, Chen, Shen, Chen, She, Jiang, Miao, Wei: "The viral oncoprotein HBx of Hepatitis B virus promotes the growth of hepatocellular carcinoma through cooperating with the cellular oncoprotein RMP." in: International journal of biological sciences, Vol. 10, Issue 10, pp. 1181-92, (2014) (PubMed).

Yang, Gu, Zheng, Li, Lian, Miao, Jiang, Wei: "RPB5-mediating protein is required for the proliferation of hepatocellular carcinoma cells." in: The Journal of biological chemistry, Vol. 286, Issue 13, pp. 11865-74, (2011) (PubMed).

Target Details for alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Alternative Name: Afp

Background:

Synonyms: Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, Afp

Tissue Specificity: Plasma.

Background: RAC-gamma serine/threonine-protein kinase, also known as protein kinase Akt-3, is an enzyme that in humans is encoded by the AKT3 gene. This gene is mapped to 1q43-q44. The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor(PDGF), insulin, and insulin-like growth factor 1(IGF1). AKT3 plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. This gene is required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands.

Molecular Weight: 69 kDa

Gene ID: 11576

UniProt: P02772

Pathways: C21-Steroid Hormone Metabolic Process