HSF2 antibody (AA 13-245)

Catalog No. ABIN5692998

This Rabbit Polyclonal antibody specifically detects HSF2 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for HSF2 antibody (AA 13-245) (ABIN5692998)

Target: HSF2 (Heat shock factor protein 2 (HSF2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-HSF2 Antibody

Binding Specificity: AA 13-245

Purpose: Anti-HSF2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-HSF2 Antibody Picoband® (ABIN5692998). Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E. coli-derived human HSF2 recombinant protein (Position: K13-D245).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Schuetz, T. J. Personal Communication. Boston, Mass. 10/3/1991. 2. Schuetz, T. J., Gallo, G. J., Sheldon, L., Tempst, P., Kingston, R. E. Isolation of a cDNA for HSF2: evidence for two heat shock factor genes in humans. Proc. Nat. Acad. Sci. 88: 6911-6915, 1991.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for HSF2

Target: HSF2 (Heat shock factor protein 2 (HSF2))

Alternative Name: HSF2

Background:

Synonyms: Heat shock factor protein 2, HSF 2, Heat shock transcription factor 2, HSTF 2, HSF2, HSTF2

Background: HSF2 (HEAT-SHOCK TRANSCRIPTION FACTOR 2), also called HEAT-SHOCK FACTOR 2, as well as the related gene HSF1, encodes a protein that binds specifically to the heat-shock element and has homology to HSFs of other species. The International Radiation Hybrid Mapping Consortium maps the HSF2 gene to chromosome 6. Mouse Hsf2 was expressed in all 3 embryonic layers at embryonic day 7.5 and that the head fold was strongly stained at embryonic day 8.5. In adults, Hsf2 expression is detected in spermatocytes and spermatogonia, but not in elongated spermatids, spermatozoa, or Sertoli cells. Hsp70i bookmarking is mediated by a transcription factor called HSF2, which binds this promoter in mitotic cells, recruits protein phosphatase-2A, and interacts with the CAPG subunit of the condensin enzyme to promote efficient dephosphorylation and inactivation of condensin complexes in the vicinity, thereby preventing compaction at this site. And Hsf2-null mice were born at the expected mendelian ratio but had brain abnormalities and meiotic and gametogenic defects in both genders.

Molecular Weight: 60 kDa

Gene ID: 3298

UniProt: Q03933