ICOS antibody (AA 21-140)

Catalog No. ABIN5693002

The Rabbit Polyclonal anti-ICOS antibody has been validated for IHC, FACS, ELISA and ICC. It is suitable to detect ICOS in samples from Human.

Quick Overview for ICOS antibody (AA 21-140) (ABIN5693002)

Target: ICOS (Inducible T-Cell Co-Stimulator (ICOS))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ICOS antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA, Immunocytochemistry (ICC)

Product Details anti-ICOS Antibody

Binding Specificity: AA 21-140

Purpose: Anti-ICOS Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ICOS Antibody Picoband® (ABIN5693002). Tested in ELISA, Flow Cytometry, IHC, ICC applications. This antibody reacts with Human.

Immunogen: E. coli-derived human ICOS recombinant protein (Position: E21-K140).

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry, 0.5-1 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. Hutloff A, Dittrich AM, Beier KC, Eljaschewitsch B, Kraft R, Anagnostopoulos I, Kroczek RA (Jan 1999). "ICOS is an inducible T-cell co-stimulator structurally and functionally related to CD28". Nature. 397(6716): 263-6. 2. Rudd CE, Schneider H (Jul 2003). "Unifying concepts in CD28, ICOS and CTLA4 co-receptor signalling". Nature Reviews. Immunology. 3 (7): 544-56.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ICOS

Target: ICOS (Inducible T-Cell Co-Stimulator (ICOS))

Alternative Name: ICOS

Background:

Synonyms: Inducible T-cell costimulator, Activation-inducible lymphocyte immunomediatory molecule, CD278, ICOS, AILIM

Tissue Specificity: Activated T-cells. Highly expressed on tonsillar T-cells, which are closely associated with B-cells in the apical light zone of germinal centers, the site of terminal B- cell maturation. Expressed at lower levels in thymus, lung, lymph node and peripheral blood leukocytes. Expressed in the medulla of fetal and newborn thymus.

Background: Inducible T-cell costimulator is an immune checkpoint protein that in humans is encoded by the ICOS gene. The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation.

Molecular Weight: 20 kDa

Gene ID: 29851

UniProt: Q9Y6W8

Pathways: Cancer Immune Checkpoints