Cytochrome C antibody (AA 2-105) (DyLight 488)

Catalog No. ABIN5693009

This anti-Cytochrome C antibody is a Mouse Monoclonal antibody detecting Cytochrome C in FACS. Suitable for Human.

Quick Overview for Cytochrome C antibody (AA 2-105) (DyLight 488) (ABIN5693009)

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytochrome C antibody is conjugated to DyLight 488

Application: Flow Cytometry (FACS)

Clone: 15F10

Product Details anti-Cytochrome C Antibody (DyLight 488)

Binding Specificity: AA 2-105

Purpose: Anti-Human Cytochrome C DyLight® 488 conjugated CYCS Antibody(monoclonal, 15F10)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Human Cytochrome C DyLight® 488 conjugated CYCS Antibody (monoclonal, 15F10) -Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.

Immunogen: E.coli-derived human Cytochrome C recombinant protein (Position: G2-E105). Human Cytochrome C shares 91% amino acid (aa) sequence identity with both mouse and rat Cytochrome C.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells1. García-Heredia JM, et al. Specific nitration of tyrosines 46 and 48 makes cytochrome c assemble a non-functional apoptosome. FEBS Lett, 2012 Jan 20. 2. Rajagopal BS, et al. The hydrogen-peroxide-induced radical behaviour in human cytochrome c-phospholipid complexes: implications for the enhanced pro-apoptotic activity of the G41S mutant. Biochem J, 2013 Dec 15. 3. Simon M, et al. Versatility of non-native forms of human cytochrome c: pH and micellar concentration dependence. J Biol Inorg Chem, 2013 Jan.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Target Details for Cytochrome C

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Alternative Name: CYCS

Background:

Synonyms: Cytochrome c, CYCS, CYC

Background: CYCS is also known as CYC, HCS or THC4. This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.

Molecular Weight: 39 kDa

Gene ID: 54205

UniProt: P99999

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity