STUB1 antibody (AA 51-303)

Catalog No. ABIN5693017

The Rabbit Polyclonal anti-STUB1 antibody has been validated for WB, ELISA, IHC, IF, FACS and ICC. It is suitable to detect STUB1 in samples from Human, Mouse and Rat.

Quick Overview for STUB1 antibody (AA 51-303) (ABIN5693017)

Target: STUB1 (STIP1 Homology and U-Box Containing Protein 1 (STUB1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STUB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-STUB1 Antibody

Binding Specificity: AA 51-303

Purpose: Anti-STUB1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-STUB1 Antibody Picoband® (ABIN5693017). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E. coli-derived human STUB1 recombinant protein (Position: R51-Y303).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. "Entrez Gene: STUB1 STIP1 homology and U-box containing protein 1". 2. Heimdal, K., Sanchez-Guixe, M., Aukrust, I., Bollerslev, J., Bruland, O., Jablonski, G. E., Erichsen, A. K., Gude, E., Koht, J. A., Erdal, S., Fiskerstrand, T., Haukanes, B. I., Boman, H., Bjorkhaug, L., Tallaksen, C. M. E., Knappskog, P. M., Johansson, S. STUB1 mutations in autosomal recessive ataxias--evidence for mutation-specific clinical heterogeneity. Orphanet J. Rare Dis. 9: 146, 2014.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for STUB1

Target: STUB1 (STIP1 Homology and U-Box Containing Protein 1 (STUB1))

Alternative Name: STUB1

Background:

Synonyms: E3 ubiquitin-protein ligase CHIP

Tissue Specificity: Highly expressed in skeletal muscle, heart, pancreas, brain and placenta. Detected in kidney, liver and lung.

Background: STUB1 (STIP1 homology and U-Box containing protein 1), also known as CHIP (C terminus of HSC70-Interacting Protein), is a human gene. This gene encodes a protein containing tetratricopeptide repeat and a U-box that functions as a ubiquitin ligase/cochaperone. The encoded protein binds to and ubiquitinates shock cognate 71 kDa protein (Hspa8) and DNA polymerase beta (Polb), among other targets. Mutations in this gene cause spinocerebellar ataxia, autosomal recessive 16. Alternative splicing results in multiple transcript variants. There is a pseudogene for this gene on chromosome 2.

Molecular Weight: 35 kDa

Gene ID: 10273

Pathways: Regulation of Hormone Metabolic Process, Response to Water Deprivation