Desmoplakin antibody (AA 1810-2092)

Catalog No. ABIN5693018

This anti-Desmoplakin antibody is a Rabbit Polyclonal antibody detecting Desmoplakin in IHC, ELISA, WB, ICC and IF. Suitable for Human, Mouse and Rat.

Quick Overview for Desmoplakin antibody (AA 1810-2092) (ABIN5693018)

Target: Desmoplakin (DSP)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Desmoplakin antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-Desmoplakin Antibody

Binding Specificity: AA 1810-2092

Purpose: Anti-Desmoplakin/DSP Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Desmoplakin/DSP Antibody Picoband® (ABIN5693018). Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E. coli-derived human Desmoplakin recombinant protein (Position: Q1810-A2092).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Choi HJ, Weis WI (2011). "Crystal structure of a rigid four-spectrin-repeat fragment of the human desmoplakin plakin domain". J. Mol. Biol. 409 (5): 800-12. 2. Green KJ, Parry DA, Steinert PM, Virata ML, Wagner RM, Angst BD, Nilles LA (Feb 1990). "Structure of the human desmoplakins. Implications for function in the desmosomal plaque". The Journal of Biological Chemistry. 265 (5): 2603-12. 2. Jonkman MF, Pasmooij AM, Pasmans SG, van den Berg MP, Ter Horst HJ, Timmer A, Pas HH (Oct 2005). "Loss of desmoplakin tail causes lethal acantholytic epidermolysis bullosa". American Journal of Human Genetics. 77 (4): 653-60.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Desmoplakin

Target: Desmoplakin (DSP)

Alternative Name: DSP

Background:

Synonyms: Desmoplakin, DP, 250/210 kDa paraneoplastic pemphigus antigen, DSP

Tissue Specificity: Isoform DPI is apparently an obligate constituent of all desmosomes. Isoform DPII resides predominantly in tissues and cells of stratified origin.

Background: Desmoplakin is a protein in humans that is encoded by the DSP gene. This gene encodes a protein that anchors intermediate filaments to desmosomal plaques and forms an obligate component of functional desmosomes. Mutations in this gene are the cause of several cardiomyopathies and keratodermas, including skin fragility-woolly hair syndrome. Alternative splicing results in multiple transcript variants.

Molecular Weight: 250-280 kDa

Gene ID: 1832

UniProt: P15924

Pathways: Cell-Cell Junction Organization