CD8 alpha antibody (AA 28-196)

Catalog No. ABIN5693154

This Rabbit Polyclonal antibody specifically detects CD8 alpha in FACS, IHC, WB and ELISA. It exhibits reactivity toward Mouse and Rat and has been mentioned in 2+ publications.

Quick Overview for CD8 alpha antibody (AA 28-196) (ABIN5693154)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD8 alpha antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), ELISA

Product Details anti-CD8 alpha Antibody

Binding Specificity: AA 28-196

Purpose: Anti-CD8 alpha/Cd8a Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CD8 alpha/Cd8a Antibody Picoband® (ABIN5693154). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E. coli-derived mouse CD8 alpha recombinant protein (Position: K28-Y196).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. Bowcock, A. M., Kavathas, P., Margolskee, R. F., Herzenberg, L., Cavalli-Sforza, L. L. An RFLP associated with pcDLeu2-14, a human T-cell differentiation antigen CD8 (Leu2) cDNA mapped to 2p12. Nucleic Acids Res. 14: 7817 only, 1986. 2. "Entrez Gene: CD8A CD8a molecule".

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-CD8 alpha antibody (ABIN5693154)

Xu, Tan, Wang, Wang, Li, Wu, Chen, Wu, Tan: "Elevated survivin expression in peripheral blood mononuclear cells is central to collateral formation in coronary chronic total occlusion." in: International journal of molecular medicine, Vol. 35, Issue 6, pp. 1501-10, (2015) (PubMed).

Zhang, Chen, Lao, Huang: "Effect of Hejie decoction on T cell immune state of chronic hepatitis B patients." in: World journal of gastroenterology, Vol. 10, Issue 10, pp. 1436-9, (2005) (PubMed).

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: Cd8a

Background:

Synonyms: T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2, CD8a, Cd8a, Lyt-2, Lyt2

Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. It is mapped to 2p11.2. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene also encodes the CD8 alpha chain isoforms.

Molecular Weight: 36 kDa

Gene ID: 12525

UniProt: P01731

Pathways: TCR Signaling