KDM2A antibody

Catalog No. ABIN5693222

This anti-KDM2A antibody is a Rabbit Polyclonal antibody detecting KDM2A in WB. Suitable for Human, Mouse and Rat.

Quick Overview for KDM2A antibody (ABIN5693222)

Target: KDM2A (Lysine (K)-Specific Demethylase 2A (KDM2A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KDM2A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-KDM2A Antibody

Purpose: Anti-FBXL11/KDM2A Antibody Picoband®

Sequence: KRTFDLEEKL HTNKYNANFV TFMEGKDFNV EYIQR

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-FBXL11/KDM2A Antibody Picoband® (ABIN5693222). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human FBXL11, which shares 97.1% and 100% amino acid (aa) sequence identity with mouse and rat FBXL11, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
1. "Entrez Gene: FBXL11 F-box and leucine-rich repeat protein 11". 2. Nagase T, Ishikawa K, Suyama M, Kikuno R, Hirosawa M, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (Jul 1999). "Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 6 (1): 63-70. 3. Winston JT, Koepp DM, Zhu C, Elledge SJ, Harper JW (Dec 1999). "A family of mammalian F-box proteins".Curr Biol. 9 (20): 1180-2.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for KDM2A

Target: KDM2A (Lysine (K)-Specific Demethylase 2A (KDM2A))

Alternative Name: KDM2A

Background:

Synonyms: Lysine-specific demethylase 2A, CXXC-type zinc finger protein 8, F-box and leucine-rich repeat protein 11, F-box protein FBL7, F-box protein Lilina, F-box/LRR-repeat protein 11, JmjC domain-containing histone demethylation protein 1A, [Histone-H3]-lysine-36 demethylase 1A, KDM2A, CXXC8, FBL7, FBXL11, JHDM1A, KIAA1004

Tissue Specificity: Widely expressed, with highest levels in brain, testis and ovary, followed by lung.

Background: Lysine-specific demethylase 2A (KDM2A) also known as F-box and leucine-rich repeat protein 11 (FBXL11) is an enzyme that in humans is encoded by the KDM2A gene. This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains at least six highly degenerated leucine-rich repeats. This family member plays a role in epigenetic silencing. It nucleates at CpG islands and specifically demethylates both mono- and di-methylated lysine-36 of histone H3. Alternative splicing results in multiple transcript variants.

Molecular Weight: 150 kDa

Gene ID: 22992

UniProt: Q9Y2K7

Pathways: Warburg Effect