VAPB antibody (AA 2-55)

Catalog No. ABIN5693292

This Rabbit Polyclonal antibody specifically detects VAPB in WB, IHC, ELISA, IF, ICC and FACS. It exhibits reactivity toward Human and Mouse.

Quick Overview for VAPB antibody (AA 2-55) (ABIN5693292)

Target: VAPB (VAMP (Vesicle-Associated Membrane Protein)-Associated Protein B and C (VAPB))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VAPB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-VAPB Antibody

Binding Specificity: AA 2-55

Purpose: Anti-VAPB Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-VAPB Antibody (ABIN5693292). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human VAPB recombinant protein (Position: A2-R55). Human VAPB shares 98.1% and 100% amino acid (aa) sequence identity with mouse and rat VAPB, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed)|1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for VAPB

Target: VAPB (VAMP (Vesicle-Associated Membrane Protein)-Associated Protein B and C (VAPB))

Alternative Name: VAPB

Background:

Synonyms: Vesicle-associated membrane protein-associated protein B/C, VAMP-B/VAMP-C, VAMP-associated protein B/C, VAP-B/VAP-C, VAPB, UNQ484/PRO983

Tissue Specificity: Ubiquitous. Isoform 1 predominates.

Background: Vesicle-associated membrane protein-associated protein B/C is a protein that in humans is encoded by the VAPB gene. The VAPB gene is found on the 20th human chromosome. The protein encoded by this gene is a type IV membrane protein found in plasma and intracellular vesicle membranes. The encoded protein is found as a homodimer and as a heterodimer with VAPA. This protein also can interact with VAMP1 and VAMP2 and may be involved in vesicle trafficking.

Molecular Weight: 27 kDa

Gene ID: 9217

UniProt: O95292

Pathways: ER-Nucleus Signaling