CHRNA3 antibody

Catalog No. ABIN5693305

The Rabbit Polyclonal anti-CHRNA3 antibody has been validated for WB and FACS. It is suitable to detect CHRNA3 in samples from Human, Mouse and Rat.

Quick Overview for CHRNA3 antibody (ABIN5693305)

Target: CHRNA3 (Cholinergic Receptor, Nicotinic, alpha 3 (Neuronal) (CHRNA3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHRNA3 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-CHRNA3 Antibody

Purpose: Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 Antibody Picoband®

Sequence: DAVLSLSALS PEIKEAIQSV KYIAENMKAQ NEAKEIQD

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 Antibody Picoband® (ABIN5693305). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CHRNA3, which shares 97.4% and 94.7% amino acid (aa) sequence identity with mouse and rat CHRNA3, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot,0.1-0.5 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. "Entrez Gene: CHRNA3 cholinergic receptor, nicotinic, alpha 3". 2. Eng CM, Kozak CA, Beaudet AL, Zoghbi HY (Apr 1991). "Mapping of multiple subunits of the neuronal nicotinic acetylcholine receptor to chromosome 15 in man and chromosome 9 in mouse". Genomics. 9 (2): 278-82. 3. Mihovilovic M, Roses AD (1991). "Expression of mRNAs in human thymus coding for the alpha 3 subunit of a neuronal acetylcholine receptor.". Exp. Neurol. 111 (2): 175-80.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CHRNA3

Target: CHRNA3 (Cholinergic Receptor, Nicotinic, alpha 3 (Neuronal) (CHRNA3))

Alternative Name: CHRNA3

Background: Neuronal acetylcholine receptor subunit alpha-3, CHRNA3, NACHRA3

Molecular Weight: 60 kDa

Gene ID: 1136

UniProt: P32297

Pathways: Synaptic Membrane