CLPX antibody (AA 337-574) (DyLight 488)

Catalog No. ABIN7354567

The Rabbit Polyclonal anti-CLPX antibody has been validated for FACS. It is suitable to detect CLPX in samples from Human.

Quick Overview for CLPX antibody (AA 337-574) (DyLight 488) (ABIN7354567)

Target: CLPX (ClpX Caseinolytic Peptidase X (CLPX))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CLPX antibody is conjugated to DyLight 488

Application: Flow Cytometry (FACS)

Product Details anti-CLPX Antibody (DyLight 488)

Binding Specificity: AA 337-574

Purpose: Anti-Human CLPX DyLight® 488 conjugated Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Human CLPX DyLight® 488 conjugated Antibody -Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.

Immunogen: E. coli-derived human CLPX recombinant protein (Position: A337-E574).

Isotype: IgG

Application Details

Application Notes: Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells1. Corydon, T. J., Wilsbech, M., Jespersgaard, C., Andresen, B. S., Borglum, A. D., Pedersen, S., Bolund, L., Gregersen, N., Bross, P. Human and mouse mitochondrial orthologs of bacterialClpX. Mammalian Genome 11: 899-905, 2000. 2. Kang, S. G., Dimitrova, M. N., Ortega, J., Ginsburg, A., Maurizi, M. R. Human mitochondrial ClpP is a stable heptamer that assembles into a tetradecamer in the presence of ClpX. J. Biol. Chem. 280: 35424-35432, 2005.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Target Details for CLPX

Target: CLPX (ClpX Caseinolytic Peptidase X (CLPX))

Alternative Name: CLPX

Background:

Synonyms: ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial, CLPX

Tissue Specificity: Higher expression in skeletal muscle and heart and to a lesser extent in liver, brain, placenta, lung, kidney and pancreas.

Background: ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial is an enzyme that in humans is encoded by the CLPX gene. This protein is a member of the family of AAA Proteins (AAA+ ATPase) and is to form the protein complex of Clp protease. The protein encoded by this gene is part of a protease found in mitochondria. This protease is ATP-dependent and targets specific proteins for degradation. The protease consists of two heptameric rings of the CLPP catalytic subunit sandwiched between two hexameric rings of the chaperone subunit encoded by this gene. Targeted proteins are unwound by this protein and then passed on to the CLPP subunit for degradation. Two transcript variants, one protein-coding and the other non-protein coding, have been found for this gene.

Molecular Weight: 39 kDa

Gene ID: 10845

UniProt: O76031