GLUD1 antibody

Catalog No. ABIN5697821

The Rabbit Polyclonal anti-GLUD1 antibody has been validated for WB, IHC, IP and ELISA. It is suitable to detect GLUD1 in samples from Human, Mouse and Rat.

Quick Overview for GLUD1 antibody (ABIN5697821)

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLUD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA

Product Details anti-GLUD1 Antibody

Purpose: GLUD1 antibody

Immunogen: glutamate dehydrogenase 1

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

HeLa cells were subjected to SDS PAGE followed by western blot with FNab03498(GLUD1 antibody) at dilution of 1:3000

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for GLUD1

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Alternative Name: GLUD1

Background:

Synonyms: Glutamate dehydrogenase 1, mitochondrial (GDH 1)|GLUD1|GLUD

Background: This gene encodes glutamate dehydrogenase, which is a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid-induced insulin secretion. It is allosterically activated by ADP and inhibited by GTP and ATP. Activating mutations in this gene are a common cause of congenital hyperinsulinism. Alternative splicing of this gene results in multiple transcript variants. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Related pseudogenes have been identified on chromosomes 10, 18 and X.

Molecular Weight: 45-55 kDa

Gene ID: 2746

UniProt: P00367

Pathways: Positive Regulation of Peptide Hormone Secretion, Warburg Effect