VEGFA antibody (AA 27-232)

Catalog No. ABIN5699617

This Rabbit Polyclonal antibody specifically detects VEGFA in WB, IHC, ELISA, IP and IF. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for VEGFA antibody (AA 27-232) (ABIN5699617)

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VEGFA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)

Product Details anti-VEGFA Antibody

Binding Specificity: AA 27-232

Purpose: VEGFA antibody

Immunogen:

Immunogen sequence: 27-232aa

Immunogen: vascular endothelial growth factor A

Isotype: IgG

Application Details

Application Notes: WB :1:200-1:2000,IP:1:200-1:2000,IF:1:10-1:100,IHC:1:50-1:500

Comment:

PC-3 cells were subjected to SDS PAGE followed by western blot with FNab09391(VEGFA antibody) at dilution of 1:800

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for VEGFA

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Alternative Name: VEGFA

Background:

Synonyms: Vascular endothelial growth factor A, long form (L-VEGF)|Vascular permeability factor (VPF)|N-VEGF|VEGFA|VEGFA|VEGF

Background: VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1(MVCD1). VEGFA has 13 isoforms with MW 16kd to 45kd. Some isoforms has homodimer form, such as VEGFA189(38kd), VEFGA110(34kd). VEGFA is a 34kd to 42kd, dimeric, disulfide-bound glycoprotein. VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids(PMID:15602010).

Molecular Weight: 23-27kDa,45kDa

UniProt: P15692

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling