Ube2t antibody

Catalog No. ABIN5700967

This Rabbit Polyclonal antibody specifically detects Ube2t in WB, ELISA, IP and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Ube2t antibody (ABIN5700967)

Target: Ube2t (Ubiquitin-Conjugating Enzyme E2T (Ube2t))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ube2t antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)

Product Details anti-Ube2t Antibody

Immunogen: ubiquitin-conjugating enzyme E2T (putative)

Isotype: IgG

Application Details

Application Notes: WB : 1:200-1:2000,IP:1:200-1:2000,IF:1:50-1:500

Comment:

Jurkat cells were subjected to SDS PAGE followed by western blot with FNab09185(UBE2T antibody) at dilution of 1:800

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Expiry Date: 12 months

Target Details for Ube2t

Target: Ube2t (Ubiquitin-Conjugating Enzyme E2T (Ube2t))

Alternative Name: UBE2T/HSPC150

Background: Synonyms:HSPC150, PIG50, UBE2T, Ubiquitin carrier protein T, Ubiquitin protein ligase T Background:The ubiquitin(Ub)-mediated protein degradation pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub to specific protein substrates. The first step requires ATP-dependent activation of the C-terminus of Ub and the assembly of multi-Ubs by Ub-activating enzyme E1. The ubiquitin-conjugating enzyme E2, catalytic(UBCc) domain, is then conjugated to Ubs, through a thiol-ester linkage between a conserved cysteine and the C-terminus of Ub, to generate an intermediate Ub-E2 complex. Then the E3, a ligase, catalyzes the transfer of Ub from E2 to the appropriate substrate. This pathway regulates many fundamental cellular processes. There are also other E2s which form thiol-ester linkages without the use of E3s as well as several UBC homologs(TSG101, Mms2, Croc-1 and similar proteins), which lack the active site cysteine essential for ubiquitination and appear to function in DNA repair pathways.

Molecular Weight: 24kDa

UniProt: Q9NPD8

Pathways: Chromatin Binding