EIF4G1 antibody

Catalog No. ABIN5702756

The Rabbit Polyclonal anti-EIF4G1 antibody has been validated for WB, IHC, ELISA, IF and IP. It is suitable to detect EIF4G1 in samples from Human, Mouse and Rat.

Quick Overview for EIF4G1 antibody (ABIN5702756)

Target: EIF4G1 (Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4G1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-EIF4G1 Antibody

Immunogen: eukaryotic translation initiation factor 4 gamma, 1

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

HeLa cells were subjected to SDS PAGE followed by western blot with FNab02724(EIF4G1 antibody) at dilution of 1:1000

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Expiry Date: 12 months

Target Details for EIF4G1

Target: EIF4G1 (Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1))

Alternative Name: EIF4G1

Background: Synonyms:EIF4F, EIF4G, EIF4GI Background:The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.

Molecular Weight: 250 kDa

Gene ID: 1981

UniProt: Q04637